Immobilization of indigenous holocellulase on iron oxide (Fe₂O₃) nanoparticles enhanced hydrolysis of alkali pretreated paddy straw

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• 作者：Ajay Kumar^a ; Surender Singh^a ; ^{ssriari@gmail.com} ; Rameshwar Tiwari^a ; Renu Goel^b ; Lata Nain^a
• 关键词：Aspergillus niger ; Solid-state fermentation ; Extracellular proteome ; Glycoside hydrolase ; LC MS/MS ; Proteomics
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：538-549
• 全文大小：1287 K
• 卷排序：96

文摘

Holocellulase from Aspergillus niger SH3 was characterized using LC-MALDI MS/MS. Total of 125 proteins including cellulases, xylanases and esterases were identified. Fe2O3 covalently immobilized enzyme showed better immobilization efficiency. Immobilized enzyme showed higher Vmax and lower Km than free enzyme. Fe2O3 immobilized enzyme gave 10 % higher saccharification yield then free enzyme.