Oct4-GFP expression during transformation of gonocytes into spermatogonial stem cells in the perinatal mouse testis

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• 作者：Ruili Li^a ; ^b ; ^{ruili.li@mcri.edu.au" class="auth_mail" title="E-mail the corresponding author} ; Amanda Vannitamby^a ; Jian-Guo Zhang^d ; ^e ; Emma L. Fehmel^f ; Bridget R. Southwell^a ; ^b ; John M. Hutson^a ; ^b ; ^c ; ^{john.hutson@rch.org.au" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gonocytes transformation ; Gonocyte migration ; Spermatogonial stem cells ; Oct4 ; Undescended testis
• 刊名：Journal of Pediatric Surgery
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：50
• 期：12
• 页码：2084-2089
• 全文大小：2074 K

文摘

In cryptorchidism perinatal failure to switch off Oct4, a germ cell (GC) marker, may lead to carcinoma in situ. We aimed to analyze Oct4 expression during mouse gonocyte transformation into spermatogonial stem cells (SSC).

Materials and methods

Testes from OG2 (Oct4-promoter driven eGFP) mice at embryonic day (E) 17 and postnatal day P0–10 underwent immunohistochemistry and immunoblotting. Antibodies against MVH, AMH, Ki67, and c-Kit were visualized by confocal microscopy. Numbers of Oct4-GFP⁺ GC and Oct4-GFP⁻ GC/tubule were counted using ImageJ. Data were analyzed using nonparametric one-way ANOVA.

Results

GC from E17-P4 were Oct4-GFP⁺. Numbers of Oct4-GFP⁻ GC/tubule increased from P6–10, whereas Oct4-GFP⁺ GC/tubule numbers remained similar between P6 and P10. Sertoli cells proliferated from E17–P10, whereas GC only proliferated from P2. Gonocytes (Oct4-GFP⁺/c-Kit⁻) central in tubules migrated to the basement membrane to become prospermatogonia (Oct4-GFP⁺/c-Kit⁻) and then SSC (Oct4-GFP⁺/c-Kit⁺) from day 4 and further developed into Oct4-GFP⁻/c-Kit⁺ at P6.

Conclusion

In Oct4-GFP mice both centrally located gonocytes and prospermatogonia located at the tubular basement membrane were Oct4-GFP⁺/c-Kit⁻ before further developing into SSC (Oct4-GFP⁺/c-Kit⁺). This indicates that Oct4 is important in gonocyte transformation into SSC. Understanding this process will aid GC tumor diagnostics and fertility potential in boys with UDT undergoing orchidopexy.