Gene expression profiling of renal dysfunction in rats with experimental cirrhosis

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• 作者：Marta Ló ; pez-Parra ; Naiara Telleria ; Esther Titos ; Anna Planagumà ; Ana Gonzá ; lez-Pé ; riz ; Vicente Arroyo ; Juan Rodé ; s and Joan Clà ; ria
• 刊名：Journal of Hepatology
• 出版年：2006
• 出版时间：August 2006
• 年：2006
• 卷：45
• 期：2
• 页码：221-229
• 全文大小：571 K

文摘

Background/Aims

Renal dysfunction is a frequent complication in advanced cirrhosis. The mechanisms underlying this complication have classically been addressed through conventional methods of study of candidate genes, but never on a genome-wide scale. In this investigation, we used microarrays to monitor global gene expression changes in the kidney of cirrhotic rats.

Methods

Renal samples were obtained from control and carbon tetrachloride-induced cirrhotic rats. RNA samples were reverse-transcribed into Cy5-labeled cDNA, combined with a Cy3-labeled reference and hybridized to oligonucleotide microarrays. Microarrays were scanned in a Genepix 4000B and data analyzed by Luminator v2.0 software.

Results

A total of 620 genes were differentially regulated (354 up and 266 down) in the cirrhotic kidney, accounting for approximately 11 % of all analyzed transcripts. Functional grouping of these genes revealed that 47 were related to the category of vascular tone and 85 to transporters/channels. Among these, we identified genes and pathways already associated with renal dysfunction as well as a new subset of genes previously unknown to participate in this complication, including a G protein-coupled receptor that binds apelin, a protein phosphatase (calcineurin B) and a number of neuropeptide receptors and growth factors.

Conclusions

These findings furnish new data for mechanistic investigation into renal dysfunction in cirrhosis.