• 作者：Josefina Ayats^a ; Estrella Martí ; n-Mazuelos^b ; ^{mcuenca-estrella@isciii.es} ; Javier Pemá ; n^c ; Guillermo Quindó ; s^d ; Fernando Sá ; nchez^e ; Julio Garcí ; a-Rodrí ; guez^f ; Josep Guarro^g ; Jesú ; s Guinea^h ; Marí ; a J. Linaresⁱ ; José ; Pontó ; n^d ; Juan L. Rodrí ; guez-Tudela^j ; Manuel Cuenca-Estrella^j ; ; Grupo de Estudio de Micologí ; a Mé ; dica de la SEIMC (GEMICOMED)^&loz ;
• 关键词：Infecció ; n fú ; ngica invasora ; Galactomanano ; Beta-glucano ; PCR diagnó ; stica ; Diagnó ; stico precoz
• 刊名：Enfermedades Infecciosas y Microbiolog¨ªa Cl¨ªnica
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：29
• 期：1
• 页码：39.e1-39.e15
• 全文大小：292 K

These guidelines are an update of recommendations for the diagnosis of invasive fungal infections by the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC) published in 2004 (Enferm Infecc Microbiol Clin. 2004, 22:32-9). In this updated version of the guidelines, a comprehensive review of the most recent diagnostic innovations and levels of evidence to recommend those diagnostic procedures are included. We first analyse conventional diagnostic methods, microscopic examination and culture, underlining their limitations which have led to the development of alternative methods, such as fungal antigen and DNA quantification. Those alternative methods of diagnosis are analysed by fungal infection. We also briefly review the methods for molecular identification of fungal species and recommendations for carrying out susceptibility tests for antifungal drugs, including reference procedures, commercial techniques and their indications.