Alteration of gene expression in MDA-MB-453 breast cancer cell line in response to continuous exposure to Trastuzumab
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- 作者：Elham Abu Sharieha ; b ; Abdulla S. Awidib ; c ; Mamoun Ahramd ; Malek A. Zihlifa ; m.zihlif@ju.edu.jo" class="auth_mail" title="E-mail the corresponding author
- 关键词：(ESR-A) ; estrogen receptor alpha ; (PR) ; progesterone ; (HER-2) ; human epidermal receptor 2 ; PCR ; POLYMERASE chain reaction ; (FDA) ; Food and Drug Administration ; (ATCC ; USA) ; American Type Culture Collection ; (FBS) ; fetal bovine serum ; (MTT) ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyl tetrazolium bromide ; (IC50) ; the inhibitory concentrations ; (ACTB) ; actin beta ; (GAPDH) ; beta-2-microglobulin ; glyceraldehyde-3-phosphate dehydrogenase ; (HPRT1) ; hypoxanthine phosphoribosyltransferase 1 ; (RPLP0) ; ri
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：415-420
- 全文大小：619 K
文摘
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The Trastuzumab resistant in MDA-MB-453 is multi factorial.
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Morphological change toward EMT was observed.
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The most up-regulated genes were IGFBP-3, TGF-β1 and EGF.
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IGFBP-3, TGF-β1 and EGF may have a critical role in Trastuzumab resistance.