The roles of the α₁-adrenergic receptor subtypes in rat embryonic implantation

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• 作者：Eszter Ducza ; Ró ; bert Gá ; spá ; r ; Attila Mihá ; lyi ; Zsolt Kormá ; nyos ; George Falkay
• 关键词：α ; ₁-adrenergic receptors ; rat ; implantation ; antisense oligonucleotide
• 刊名：Fertility and Sterility
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：91
• 期：4
• 页码：1224-1229
• 全文大小：308 K

文摘

Objective

To focus on the possible roles of α₁-adrenergic receptors (α₁-ARs) in rat embryonic implantation.

Design

Laboratory study.

Setting

Animal and pharmacology laboratory at Department of Pharmacodynamics and Biopharmacy, University of Szeged, Hungary.

Animal(s)

Pregnant and nonpregnant Sprague-Dawley rats.

Intervention(s)

Uterus tissues were collected during the peri-implantation period.

Main Outcome Measure(s)

We used a reverse transcription–polymerase chain reaction (RT-PCR) and Western blotting to demonstrate the expressions of mRNAs and the protein expressions of the α₁-AR subtypes in the early-pregnant uterus. Electric field stimulation was applied to test the pharmacologic reactivity of the α_1A-AR, and the physiologic role of this receptor was tested in a knock-down transformed animal model using an antisense oligonucleotide that elicits sequence-selective inhibition of the α_1A-AR gene expression.

Result(s)

The presence of all α₁-AR subtypes (α_1A, α_1B, and α_1D) was proved, with a predominance of α_1A-AR. The maximal expression of the α_1A-AR was attained on the day of implantation. The selective α_1A antagonist 5-methylurapidil inhibited the contraction in a dose-dependent manner. The number of implantation sites was decreased (75 % ) in the α_1A-AR knock-down transformed rats.

Conclusion(s)

We assume that the α_1A-AR predominance plays a crucial role in embryonic implantation in the rat.