α-TOC-loaded niosome was performed by green method using span60 and tween60 as surfactants. By increasing the HLB value of the surfactant mixture, EE of α-TOC increased up to 98.91%. Presence of DCP and Chol improved the stability of α-TOC-loaded niosome. α-TOC-loaded niosome had sustained release profile in the simulated gastric and intestinal fluid.