Self-validating lab-on-a-chip for monitoring enzyme-catalyzed biological reactions
详细信息 查看全文
- 作者：Bergoi Ibarluceaa ; 1Author Vitae ; Xavier Munoz-BerbelaAuthor Vitae ; Pedro Ortiza ; 2Author Vitae ; Stephanus Bü ; ttgenbachbAuthor Vitae ; Cé ; sar Ferná ; ndez-Sá ; ncheza ; cesar.fernandez@imb-cnm.csic.es" class="auth_mail" title="E-mail the corresponding author ; cesar.fernandez@csic.es" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Andreu Lloberaa ; b ; andreu.llobera@imb-cnm.csic.es" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：ABTS ; 2 ; 2&prime ; -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ; LoC ; lab-on-a-chip ; GOx ; glucose oxidase ; HRP ; horseradish peroxidase ; LOD ; limit of detection ; MIR ; multiple internal reflection ; PBS(T) ; phosphate buffer saline (tween 20) ; PDMS ; polydimethylsiloxane ; PECVD ; plasma-enhanced chemical vapor deposition ; PGMEA ; propylene glycol methyl ether acetate ; PVA ; polyvinyl alcohol ; RT ; room temperature ; TEA ; triethylamine ; TESU ; 11-triethoxysilyl undecanal ; TIR ; total internal reflection
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：237
- 期：Complete
- 页码：16-23
- 全文大小：1525 K
文摘
- •
A lab-on-a-chip with dual detection of enzymatic reactions is presented.
- •
A biofunctionalized mixer/reactor, and a measurement chamber is integrated.
- •
Optical (colorimetric) and electrochemical (amperometric) detection approaches are implemented.
- •
Glucose is detected in only 60 s at concentrations in the micromolar range.
- •
The dual detection allows for self-validation, ideal for biomedical applications.