Prostaglandin H synthase kinetics in the two-phase aqueous-micellar system
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- 作者：Olga A. Ponomareva ponomareva489@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Nikita A. Trushkin hydrodiction@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ivan S. Filimonov i.s.filimonov.msu@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Alexandr V. Krivoshey krivoshey.alexr@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Vladimir I. Barkhatov barkhatovv@yandex.ru" class="auth_mail" title="E-mail the corresponding author ; Sergey I. Mitrofanov mitroser04@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Petr V. Vrzheshch ; biocentr@list.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：PGHS ; prostaglandin H synthase ; AA ; arachidonic acid ; COX reaction ; cyclooxygenase reaction ; POX reaction ; peroxidase reaction ; KM ; Michaelis constant in the Michaelis-Menten equation ; Vm ; the maximum enzymatic reaction rate in the Michaelis-Menten equation ; IC50 ; the half maximal inhibitory concentration ; TMPD ; N ; N ; N&prime ; N&prime ; -Tetramethyl-p-phenylenediamine ; disodium EDTA ; disodium dihydrogen ethylenediamine tetraacetate ; DEDTC ; diethyldithiocarbamate ; DEAE Sepharose ; Diethylaminoethyl Se
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1858
- 期：9
- 页码：2199-2207
- 全文大小：644 K
文摘
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Tween-20 micelle radius in the reaction mixture equals 4–5 nm.
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The distribution ratio of AA between the aqueous phase and the micelles P ≫ 1000
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“True” KMOx equals 9.8 μM O2 in aqueous phase or 0.0074 bar in the gaseous phase.
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“True” KMAA equals 5400 μM AA in the micelles or 5400/P μM AA in the aqueous phase.