Recent Advances in Deciphering the Structure and Molecular Mechanism of the AAA + ATPase N-Ethylmaleimide-Sensitive Factor (NSF)
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- 作者：Minglei Zhao ; Axel T. Brunger ; brunger@stanford.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：NSF ; N-ethylmaleimide-sensitive factor ; SNARE ; soluble N-ethylmaleimide-sensitive factor attachment protein receptor ; SNAP ; soluble N-ethylmaleimide-sensitive factor attachment protein ; cryo-EM ; cryo-electron microscopy ; EM ; electron microscopy
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：8 May 2016
- 年：2016
- 卷：428
- 期：9
- 页码：1912-1926
- 全文大小：2763 K
文摘
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Recent progress in understanding the structure and molecular mechanism of NSF has made it one of the best-characterized Type II AAA + ATPases.
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Cryo-electron microscopy is a powerful method for structural characterization of Type II AAA + ATPases. However, imposing symmetry should be avoided during cryo-electron microscopy reconstruction whenever possible.
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Although significant progress has been made in elucidating the mechanism of NSF, there are still many remaining questions.