It is a simple and efficient method for discrimination plant biotypes based on volatile compounds in leaves using HS-SPME/GC-MS and chemometric analysis The method was successfully applied to real samples of Eugenia uniflora L. biotypes and under the optimized extraction conditions 33 VOCs were identified. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) provided a suitable tool to differentiate the volatile profiles in plants leaves with different kinds of fruit color biotypes.