Transcriptional regulation of nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase in murine hepatoma cells by 6-(methylsufinyl)hexyl isothiocyanate, an active principle of wasabi (Eutrema wasabi Maxim)
详细信息 查看全文
- 作者：Hou ; De-Xing ; Fukuda ; Masahiro ; Fujii ; Makoto ; Fuke ; Yoko
- 关键词：Wasabi ; 6-(Methylsufinyl)hexyl isothiocyanate ; Nicotinamide adenine dinucleotide phosphate ; quinone oxidoreductase ; Induction ; Reverse transcription-polymerase chain reaction ; Transfection
- 刊名：Cancer Letters
- 出版年：2000
- 出版时间：December 20, 2000
- 年：2000
- 卷：161
- 期：2
- 页码：195-200
- 全文大小：174 K
文摘
Wasabi is a very popular pungent spice in Japan. This study examined the ability of 6-(methylsufinyl)hexyl isothiocyanate (6-MITC), an active principle of wasabi, to induce the cellular expression of nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase (QR) in Hepa 1c1c7 cells. The cells were treated with various concentrations of 6-MITC, and were then assessed for cell growth, QR activity and QR mRNA expression. The induction of QR activity and QR mRNA expression was time- and dose-responsive over a narrow range of 0.1–5 μM, with declining induction at higher concentrations due to cell toxicity. Furthermore, transfection studies demonstrated that the induction of transcription of the QR gene by 6-MITC involved an antioxidant/electrophile-responsive element (ARE/EpRE) activation. Our results suggest a novel mechanism by which dietary wasabi 6-MITC may be implicated in cancer chemoprevention.