BrMOZPhC, a novel coumarin type reagent for the fluorescent derivatisation of carboxylic acids
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- 作者：Gikas ; Evagelos ; Parissi-Poulou ; Maria ; Kazanis ; Michael ; Vavagianis ; Andreas
- 关键词：Fluorescence derivatisation ; Coumarin ; Bromomethyl ; HPLC
- 刊名：Analytica Chimica Acta
- 出版年：2003
- 出版时间：August 18, 2003
- 年：2003
- 卷：489
- 期：2
- 页码：153-163
- 全文大小：151 K
文摘
A new fluorescent reagent was synthesized in order to be used as a fluorescent probe in analytical HPLC derivatisation reactions. Briefly, 2,4-dimethoxyaniline was acylated and demethylated and the resulting diphenol reacted with α-bromoacetoacetate in the presence of conc. sulfuric acid (the Von Pechmann reaction). The resulting acetamido coumarin was hydrolyzed and the aminohydroxy derivative cycled to form the corresponding bromo-coumarinoxazine. The analytical reaction was performed with butyric acid as model compound and catalyzed by 18-crown-6 ether. The calibration curve constructed was characterized by good linearity whereas the precision of the reaction gave an R.S.D. values below 3 % . The reaction applied to the separation of five aliphatic acids.