Solid-phase microextraction and chiral HPLC analysis of ibuprofen in urine
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- 作者：de Oliveira ; Anderson Rodrigo Moraes ; Cesarino ; Evandro José ; Bonato ; Pierina Sueli
- 关键词：Solid-phase microextraction ; Ibuprofen enantiomers ; Enantiomeric separation ; Urine
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2005
- 出版时间：April 25, 2005
- 年：2005
- 卷：818
- 期：2
- 页码：285-291
- 全文大小：122 K
文摘
A simple and rapid solid-phase microextraction method was developed for the enantioselective analysis of ibuprofen in urine. The sampling was made with a polydimethylsiloxane-divinylbenzene coated fiber immersed in the liquid sample. After desorptioning from the fiber, ibuprofen enantiomers were analyzed by HPLC using a Chiralpak AD-RH column and UV detection. The mobile phase was made of methanol–pH 3.0 phosphoric acid solution (75:25, v/v), at a flow rate of 0.45mL/min. The mean recoveries of SPME were 19.8 and 19.1 % for (−)-R-ibuprofen and (+)-(S)-ibuprofen, respectively. The method was linear at the range of 0.25–25μg/mL. Within-day and between-day assay precision and accuracy were below 15 % for both ibuprofen enantiomers at concentrations of 0.75, 7.5 and 20μg/mL. The method was tested with urine quality control samples and human urine fractions after administration of 200mg rac-ibuprofen.