文摘
Exposure to ultraviolet B (UVB) radiation induces local and systemic changes of immunological functions in mammals. Very little is known of the effects of UV radiation on the immune system of lower vertebrates. In this study adult cyprinid fish, roach, were exposed to a single dose of UVB. Fish were sampled 1, 3, 7 and 14 days after exposure and leukocytes from spleen and anterior kidney were separated by density gradient. Natural cytotoxic cell (NCC) activity by cells from the kidney were assayed and mitogen stimulated cultures of splenic lymphocytes were performed. Cells were cultured for 3 days and proliferative responses monitored by incorporation of radioactive thymidine.