Identification and characterization of homologues of the Exocyst component Sec10p

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• 作者：Guo ; Wei ; Roth ; Dagmar ; Gatti ; Evelina ; De Camilli ; Pietro ; et. al.
• 关键词：Exocytosis ; Exocyst ; Sec10p
• 刊名：FEBS Letters
• 出版年：1997
• 出版时间：March 10, 1997
• 年：1997
• 卷：404
• 期：2-3
• 页码：135-139
• 全文大小：1.09 M

文摘

The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23 % amino acid identity to yeast Sec10p and 37 % identity to a C. elegans protein found in the database. Northern and Western blot analyses indicate that hSec10 has a broad tissue distribution. Immunofluorescence staining of COS cells cotransfected with hSec10p and a mammalian Sec8p demonstrates that these two proteins have an identical distribution in the cell including a localization in the peripheral cytoplasm. These data suggest that hSec10p is a component of the mammalian counterpart of the yeast Exocyst complex essential for post-Golgi traffic.