文摘
We used mRNA display and an e10FnIII scaffold to find state-specific binders of Ras: RasIn1 and RasIn2. Rasin1 and RasIn2 are stable and functional inside the cells. RasIn1 and RasIn2 bind K-Ras, H-Ras, and oncogenic mutants, but not Rap1B or Arf1 homologs. RasIn2 has similar affinity for Ras as the Ras-binding domain of Raf1, the canonical binding partner of Ras.