Liquid lipases for enzymatic concentration of n-3 polyunsaturated fatty acids in monoacylglycerols via ethanolysis: Catalytic specificity and parameterization

详细信息    查看全文

• 作者：Yongjin He^a ; ^b ; Jingbo Li^a ; Sitharam Kodali^a ; Thomas Balle^d ; Bilian Chen^b ; ^c ; Zheng Guo^a ; ^{guo@eng.au.dk}
• 关键词：Liquid lipase ; Monoacylglycerols (MAGs) ; n-3 polyunsaturated fatty acids (n-3 PUFAs) ; Catalytic specificity ; Concentration effect ; Candida antarctica lipase A (CAL-A)
• 刊名：Bioresource Technology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：224
• 期：Complete
• 页码：445-456
• 全文大小：743 K
• 卷排序：224

文摘

Five liquid lipases are examined for catalytic ethanolysis of fish oil to produce MAGs. Lipase TL, Eversa Transform & NS afford faster reaction/higher MAGs yield (>30%). CAL-A gives MAGs containing the highest n-3 PUFAs (88%), followed by lipase NS (81%). 13C NMR analysis indicates CAL-A/lipase Eversa Transform are non-regiospecific. Higher activity/lower operation cost endows liquid lipases with competitive advantage.