Bioindicators and biomarkers were analyzed in chub liver and gills along Sava River.
CuZnSOD, GSH, HSP90 and MTs in the gills were highest in Zagreb.
Results suggest that Zagreb had higher concentrations of metals in water (iron).
Higher levels of GPx and CAT were found in Sremska Mitrovica and Belgrade.
Total environment influences metabolic profile and bioindicators level.