刊名:Journal of Pharmaceutical and Biomedical Analysis
出版年:2017
出版时间:5 February 2017
年:2017
卷:134
期:Complete
页码:333-339
全文大小:2035 K
卷排序:134
文摘
Ligand displacement assay based on DNAzyme-catalyzed CL detection was developed for screening anticancer drug via stabilizing promoter DNA. Alkaloid/G4 DNA interaction of end-stacking mode between alkaloid and Pu22 DNA demonstrated the feasibility of the proposed mechanism. Microfluidic-based CL approach showed the advantage of automatic, high throughput with just trace amount of sample and reagent consumption.