Enhanced production of insect raw-starch-digesting alpha-amylase accompanied by high erythritol synthesis in recombinant Yarrowia lipolytica fed-batch cultures at high-cell-densities
Raw-starch digesting α-amylase from rice weevil was produced in Y. lipolytica host. Intermittent fed-batch cultures at high-cell-densities were conducted. The peak activity of the enzyme was improved over 160-fold. High erythritol and moderate citric acid titers accompanied the process. Carbon and nitrogen fluxes distribution during the process was calculated.