Generation of highly enriched V2a interneurons from mouse embryonic stem cells
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- 作者：Nisha R. Iyera ; James E. Huettnerb ; Jessica C. Buttsa ; Chelsea R. Browna ; Shelly E. Sakiyama-Elberta ; sakiyama@wustl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CPG ; central pattern generator ; Cyc ; cyclopamine ; DAPT ; N-{N-(3 ; 5-difluorophenacetyl-L-alanyl))-(S)-phenylglycine-t-butyl-ester ; EB ; embryoid body ; EPSC ; excitatory post synaptic current ; ESC ; embryonic stem cell ; FACS ; Fluorescence-activated cell sorting ; IN ; interneuron ; MAP2 ; microtubule associated protein 2 ; MN ; motor neuron ; PAC ; puromycin N-acetyltransferase ; pMN ; progenitor motor neuron ; Pur ; purmorphamine ; Puro ; puromycin ; RA ; retinoic acid ; SCI ; spinal cord injury ; Shh ; sonic hedgehog
- 刊名：Experimental Neurology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：277
- 期：Complete
- 页码：305-316
- 全文大小：2267 K
文摘
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Antibiotic selection of transgenic mouse ESCs yields highly enriched V2a interneurons.
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Purity of ESC-derived V2a IN culture persists through glutamatergic maturation.
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Electrophysiological properties of selected V2a INs are similar to endogenous cells.
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Selected ESC-derived V2a INs form synapses with motor neurons when co-cultured.