Mutations in exocyst complex subunit SEC6 gene impaired polar auxin transport and PIN protein recycling in Arabidopsis primary root
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- 作者：Xiaoyun Tan ; Yihong Feng ; Yulong Liu ; Yiqun Bao ; baoyiqun@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：ABRC ; Arabidopsis biological resource centre ; BFA ; Brefeldin A ; CLSM ; confocal laser scanning microscopy ; DAG ; days after germination ; GUS ; β-glucuronidase ; IAA ; indole-3-acetic acid ; NAA ; 1-napthalene acetic acid ; PM ; plasma membrane ; TEM ; transmission electron microscopy ; 2 ; 4-D ; 2 ; 4-dichlorophenoxy
- 刊名：Plant Science
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：250
- 期：Complete
- 页码：97-104
- 全文大小：3542 K
文摘
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In exocyst subunit SEC6 loss-of-function mutants, PRsec6-1 and PRsec6-2, the primary root growth and lateral root initiation were retarded.
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PRsec6 primary root growth was sensitive to exogenous auxin NAA but not 2,4-D.
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Recycling of PIN1 and PIN2 proteins from the BFA compartments to the PM was delayed in PRsec6 mutant.
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In PRsec6 mutant, vesicles accumulated in the primary root tip cells, especially closed to the PM, suggesting that exocytosis was arrested by SEC6 mutation.
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In short, SEC6 play a role in PIN protein recycling, primary root growth and lateral root initiation.