Monocyty z ekspresją Tie-2 u chorych na przewlekłą białaczkę limfocytową

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• 作者：Agnieszka Bojarska-Junak¹ ; ^{abojarskajunak@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Piotr Grundszok¹ ; Małgorzata Waldowska¹ ; Justyna Woś¹ ; Sylwia Chocholska² ; Iwona Hus³ ; Wioleta Kowalska¹ ; Katarzyna Gęca¹ ; Waldemar Tomczak² ; ⁴ ; Jacek Roliński¹
• 关键词：przewlekła białaczka limfocytowa ; Tie-2 ; monocyty ; TEM ; CD38 ; ZAP-70
• 刊名：Acta Haematologica Polonica
• 出版年：2016
• 出版时间：July-September 2016
• 年：2016
• 卷：47
• 期：3
• 页码：211-218
• 全文大小：1475 K

文摘

In peripheral blood, monocytes form a heterogeneous population of cells. One particular subset of circulating monocytes is expressing the angiopoietin receptor Tie-2 (Tie2-expressing monocyte; TEM). TEM are characterized by tumor promoting properties. However, the role of TEM in chronic lymphocytic leukemia (CLL) immunopathogenesis remains undefined.

Material and Methods

Here, we evaluated the monocytes with Tie-2 expression (CD14⁺Tie-2⁺) in peripheral blood of CLL patients (n = 55) and normal subjects (n = 15) by flow cytometry. We investigated possible associations between TEM and poor prognostic factors such as CD38 or ZAP-70 expression, Rai stage and unfavorable cytogenetic abnormalities. Moreover, we investigated the association of TEM percentage with CD14⁺⁺CD16⁺ monocytes and Treg percentages.

Results

We found that CLL patients had a higher percentage of CD14⁺Tie-2⁺ monocytes compared to normal controls. The percentage of TEM was positively associated with ZAP-70 expression as well as with unfavourable cytogenetic changes: del(17p) and/or del(11q). The frequency of TEM increased with the disease stage. We showed no correlation between the percentage of TEM and CD38 expression. The percentage of TEM at diagnosis was associated with white blood cell count as well as with the percentages of CD19⁺CD5⁺ lymphocytes and Tregs. The majority of CD14⁺Tie-2⁺ cells belonged to the intermediate monocytes subset (CD14⁺⁺CD16⁺) while fewer of them were among the classical (CD14⁺⁺CD16⁻) or non-classical monocyte (CD14⁺CD16⁺⁺) subsets. TEM and CD14⁺⁺CD16⁺ monocytes have a proangiogenic activity, suppress T-cell activation and promote Treg expansion. The results suggest that monitoring of TEM number and function may provide useful information in determining disease activity.