Use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity
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- 作者：Keener ; W.K. ; Watwood ; M.E. ; Schaller ; K.D. ; Walton ; M.R. ; Partin ; J.K. ; Smith ; W.A. ; Clingenpeel ; S.R.
- 关键词：Toluene oxygenases ; Aquifer bacteria ; Enzyme indicators ; Enzyme inhibitors ; Toluene
- 刊名：Journal of Microbiological Methods
- 出版年：2001
- 出版时间：September, 2001
- 年：2001
- 卷：46
- 期：3
- 页码：171-185
- 全文大小：297 K
文摘
In whole-cell studies, two alkynes, 1-pentyne and phenylacetylene, were selective, irreversible inhibitors of monooxygenase enzymes in catabolic pathways that permit growth of bacteria on toluene. 1-Pentyne selectively inhibited growth of Burkholderia cepacia G4 (toluene 2-monooxygenase [T2MO] pathway) and B. pickettii PKO1 (toluene 3-monooxygenase [T3MO] pathway) on toluene, but did not inhibit growth of bacteria expressing other pathways. In further studies with strain G4, chromogenic transformation of α,α,α-Trifluoro-m-cresol (TFC) was irreversibly inhibited by 1-pentyne, but the presence of phenol prevented this inhibition. Transformation of catechol by G4 was unaffected by 1-pentyne.