Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells

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• 作者：A.E. Greijer¹ ; O. Ramayanti¹ ; S.A.W.M. Verkuijlen ; Z. Novalić ; H. Juwana ; J.M. Middeldorp ; ^{j.middeldorp@vumc.nl}
• 关键词：Epstein-Barr virus ; Quantitative RT-PCR ; RNA expression profile
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：24-33
• 全文大小：1234 K
• 卷排序：241

文摘

Multi-primed quantitative RT-PCR is suitable for accurate quantification of RNA molecules enabling standardisation. Viral RNA profiling revealed the expected RNA expression patterns according to the latency program and presence of reactivating EBV. Quantification of 16 viral targets enables monitoring of EBV behaviour in malignancies during therapy as biomarkers for therapy response.