Non-ionic polysorbate surfactants: Alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for production of extracellular MCL-PHA depolymerases

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• 作者：Bong Su Seo ; Do Young Kim ; Yu-Yang Ni ; Kwang-Hee Son ; Ho-Yong Park ; Young Ha Rhee
• 关键词：Pseudomonas alcaligenes LB19 ; Medium-chain-length poly(3-hydroxyalkanoate) depolymerase ; MCL-PHA ; Polysorbate surfactants ; Tween compounds
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：121
• 期：Complete
• 页码：47-53
• 全文大小：388 K

文摘

The potential of non-ionic polysorbate surfactants as alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for the production of diverse bacterial MCL-PHA depolymerases was evaluated. When grown with corn oil as the sole carbon substrate, Pseudomonas alcaligenes LB19 preferentially produced lipolytic enzymes, but its MCL-PHA depolymerase was not induced by the substrate. However, the results of activity staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis clearly revealed that Tween 20 induced simultaneous production of lipolytic enzymes and the MCL-PHA depolymerase with the molecular mass (26.5 kDa) of P. alcaligenes LB19, which has been previously identified. Moreover, the co-production of two functionally distinct hydrolytic enzymes induced by Tween 20 was commonly observed in various Gram-positive and Gram-negative bacteria that were fed the substrate. Thus, it is expected that non-ionic polysorbate surfactants including Tween 20 can be widely exploited as promising universal substrates for the facile and efficient production of diverse MCL-PHA depolymerases.