Analysis and determination of diterpenoids in unprocessed and processed Euphorbia lathyris seeds by HPLC¨CESI-MS

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• 作者：Xiao-Rong Hou^a ; Lei-Lei Wan^a ; Zha-Jun Zhan^a ; Cheng-Ping Li^b ; Wei-Guang Shan^a ; ^{swg@zjut.edu.cn}
• 关键词：Toxic and potent Chinese materia medica (T/PCMM) ; High-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC&ndash ; ESI-MS) ; Euphorbia lathyris ; Diterpenoids
• 刊名：Journal of Pharmaceutical Analysis
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：1
• 期：3
• 页码：197-202
• 全文大小：724 K

文摘

Euphorbia lathyris (Caper spurge) is a toxic and potent Chinese materia medica (T/PCMM). This study sought a method for identifying five diterpenoids (Euphorbia factors L₁?L₃, L_7a and L₈) with the spectra of UV and mass, quantifying three diterpenoids L₁, L₂, and L₈ in crude extracts of unprocessed and processed E. lathyris seeds by liquid chromatography/electrospray ionization mass spectrometry (LC¨CESI-MS). The analysis was achieved on an Agilent Eclipse XDB-C18 column (4.6 mm¡Á150 mm i.d., 5 ¦Ìm) with an isocratic elution with a mobile phase consisting of water and acetonitrile at a flow rate of 0.25 mL/min at column temperature of 30 ¡ãC and UV detection was set at 272 nm. An ESI source was used with a positive ionization mode. The calibration curve was linear in the ranges of 9.9?9 ¦Ìg/mL for Euphorbia factor L₁, 3.8?0.5 ¦Ìg/mL for Euphorbia factor L₂, and 1.0?0.6 ¦Ìg/mL for Euphorbia factor L₈. The average recoveries (n=6) of three diterpenoids were 98.39 % , 91.10 % and 96.94 % , respectively, with RSD of 2.5 % , 2.4 % and 2.1 % , respectively. The contents of the three diterpenoids in processed E. lathyris seeds were 3.435, 1.367 and 0.286 mg/g, respectively, which decreased more sharply than those in unprocessed E. lathyris seeds which were 4.915, 1.944 and 0.425 mg/g, respectively. The method is simple, accurate, reliable and reproducible, and it can be applied to control the quality of unprocessed and processed E. lathyris seeds.