Counter-current CO2 extraction of fat from soy skim is studied for the first time.
The highest fat extraction was achieved at 25.16 MPa and 50 °C (supercritical CO2).
The fat content of soy skim was reduced from 4.4 to 0.7%.
Counter-current CO2 settings did not affect protein structure and content.
Supercritical CO2 treated samples showed increased protein solubility in aqueous media at pH 2–10.