Antityrosinase, antioxidative activities, and brine shrimp lethality of ethanolic extracts from Protium serratum (Wall. ex Colebr.) Engl.

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• 作者：Patcharawan Tanamatayarat ; ^{patcharawan_070@outlook.co.th" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antityrosinase ; Protium serratum ; Antioxidant ; Phytochemistry ; Free radical
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：12
• 页码：1050-1055
• 全文大小：343 K

文摘

To evaluate all parts of Protium serratum (Wall. ex Colebr.) Engl. (P. serratum) for their phytochemistry and biological activities including antityrosinase, antioxidant, and brine shrimp lethality.

Methods

Nine ethanolic extracts from different P. serratum parts such as twig, whole fruit, pericarp, and root were investigated for their phytochemical screening and biological activities in terms of tyrosinase inhibition, antioxidant against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, and lethality to brine shrimp larvae.

Results

Phytochemical screening also revealed the presence of flavonoids, condensed tannins, alkaloids, triterpenoids, steroids, and sugars in P. serratum. The root extract was the most effective for antityrosinase activity with IC₅₀ of (21.63 ± 0.31) μg/mL, and the leaf extract exhibited the highest antioxidation activity using 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging methods, with IC₅₀ of (4.34 ± 0.09) and (119.80 ± 1.01) μg/mL, respectively. The essential oil extracted from the whole fruit displayed the highest toxicity against brine shrimp, with LC₅₀ of (3.57 ± 1.82) μg/mL.

Conclusions

This study indicates that ethanolic extracts from each P. serratum part have differences in phytochemistry and biological activities (antityrosinase, antioxidant, and brine shrimp lethality). Some parts of the plant should be considered in the further study.