GC/MS and LC/MS analysis, and antioxidant and antimicrobial activities of various solvent extracts from Mirabilis jalapa tubers

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• 作者：Mohamed Hajji ; Raoudha Jarraya ; Imen Lassoued ; Ons Masmoudi ; Mohamed Damak ; Moncef Nasri
• 关键词：Mirabilis jalapa tubers ; Antioxidant activity ; Antimicrobial activity ; Gas chromatography/mass spectrometry ; Liquid chromatography/mass spectrometry
• 刊名：Process Biochemistry
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：45
• 期：9
• 页码：1486-1493
• 全文大小：411 K

文摘

The antioxidant and antimicrobial activities of various solvent extracts from Mirabilis jalapa tubers (MJT) were investigated using various in vitro assays. The total phenolic and flavonoid contents varied from 21.45 to 364.6 mg gallic acid equivalent (GAE)/g dried extract and 5.2 to 71.6 mg quercetin/g dried extract, respectively. Water extract of MJT was the most potent antioxidant in all assays used, followed by methanol extract. The five solvent extracts were screened for antibacterial and antifungal activities. Water extract was the most effective with minimum inhibitory concentration <200 μg/ml against Staphylococcus aureus, Micrococcus luteus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus and Enterococcus faecalis. Only water extract showed antifungal activity against Aspergillus niger, Fusarium solani, Fusarium oxysporium and Fusarium granularium. GC/MS analysis of MJT dichloromethane and methanol extracts showed that oleic acid and β-sitosterol were, respectively, the major compounds. LC/MS analysis of the aqueous extract showed a high content of flavanol and flavonol compounds. Phenolic acids such as ferulic and caffeic acid were also detected.

To our knowledge, this is the first report on the chemical composition, and antioxidant and antimicrobial activities of phenolic extracts from M. jalapa tubers (MJT). The results of the present work indicate that MJT extracts could be used as natural antioxidant and antimicrobial agents in the food preservation and human health.