NO trapping in biological systems with a functionalized zeolite network
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- 作者：E. van Faassen and A. Vanin
- 关键词：Nitric oxide ; Iron– ; dithiocarbamate complexes ; EPR ; Spin trapping ; Zeolite ; NO sensor
- 刊名：Nitric Oxide
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：15
- 期：3
- 页码：233-240
- 全文大小：243 K
文摘
Zeolite-Y powder has been functionalized with ferric iron–diethyldithiocarbamate complexes and applied to trap nitric oxide radicals in liquids and biological systems. The complexes have been assembled in situ in the pores of zeolite-Y and act as traps for nitric oxide radicals. The resulting mononitrosyl–iron complexes form a mixture of diamagnetic ferric and paramagnetic ferrous complexes. The yield of trapped NO may be determined ex situ using electron paramagnetic resonance. The material may be anchored on solid surfaces, mixed into a composite or compressed into small pellets. The material was used to detect endogenous NO in endothelial cell cultures and spinach leaves. The sensitivity of the functionalized zeolite is significantly better than that achieved in conventional trapping of NO with iron–diethyldithiocarbamate complexes.