Performance of MRS in metabolic profiling of the lumbar spinal cord in rat and mice

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• 作者：Saeedeh Amirmohseni ; Lydia Wachsmuth ; Nathalie Just ; Cornelius Faber ; ^{faberc@uni-muenster.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MRS ; magnetic resonance spectroscopy ; SC ; spinal cord ; tCho ; total choline ; tCr ; total creatine ; Glu ; glutamate ; GABA ; γ-aminobutyric acid ; Gly ; glycine ; SCI ; spinal cord injury ; L1 ; first lumbar vertebrate ; CRLB ; Cramer&ndash ; Rao lower bounds ; Ala ; alanine ; PCr ; phospho-creatine ; Gln ; glutamine ; GSH ; glutathione ; Ins ; inositol ; Lac ; lactate ; NAA ; N-acetylaspartate ; NAAG ; N-acetylaspartylglutamate ; SNR ; signal to noise ratio ; OVS ; outer volume suppression
• 刊名：Magnetic Resonance Imaging
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：34
• 期：8
• 页码：1155-1160
• 全文大小：830 K

文摘

In vivo magnetic resonance spectroscopy (MRS) of the rodent spinal cord (SC) is technically challenging. We investigated the feasibility of MRS in the SC of both rat and mice, by comparing the spectral characteristics. We assessed possible species dependent differences in the suitability for non-invasive metabolite monitoring in the SC.

Materials and methods

MR spectra using a STEAM sequence were acquired from a rectangular voxel in lumbar SC of rats and mice, after a two-step shim procedure.

Results

In addition to total choline (tCho) and total creatine (tCr), seven and eleven metabolites were reliably detected in rats and mice, respectively. No significant differences were observed in metabolite concentrations or spectral characteristics between species.

Conclusion

Identification and quantification of major metabolites including the neurotransmitters γ-aminobutyric acid (GABA) and glycine (Gly) in the SC was successful in both rat and mice showing that investigation of SC neurochemical profiles is feasible in both species.