Conformational plasticity at the IgE-binding site of the B-cell receptor CD23

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• 作者：Balvinder Dhaliwal ; Marie O.Y. Pang ; Daopeng Yuan ; Norhakim Yahya ; Stella M. Fabiane ; James M. McDonnell ; Hannah J. Gould ; Andrew J. Beavil ; Brian J. Sutton
• 关键词：IgE ; immunoglobulin E ; ADAM10 ; a disintegrin and metalloproteinase domain-containing protein 10 ; NOE ; nuclear Overhauser effect ; PDB ; Protein Data Bank
• 刊名：Molecular Immunology
• 出版年：2013
• 出版时间：31 December, 2013
• 年：2013
• 卷：56
• 期：4
• 页码：693-697
• 全文大小：1021 K

文摘

IgE antibodies play a central role in allergic disease. They recognize allergens m>viam> their Fab regions, whilst their effector functions are controlled through interactions of the Fc region with two principal cell surface receptors, Fc?RI and CD23. Crosslinking of Fc?RI-bound IgE on mast cells and basophils by allergen initiates an immediate inflammatory response, while the interaction of IgE with CD23 on B-cells regulates IgE production. We have determined the structures of the C-type lectin ¡°head¡± domain of CD23 from seven crystal forms. The thirty-five independent structures reveal extensive conformational plasticity in two loops that are critical for IgE binding.