Biochemical and full genome sequence analyses of clinical Vibrio cholerae isolates in Mexico reveals the presence of novel V. cholerae strains

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• 作者：José ; Alberto Dí ; az-Quiñ ; onez^a ; ^b ; ^{alberto.diaz@salud.gob.mx" class="auth_mail" title="E-mail the corresponding author} ; Irma Herná ; ndez-Monroy^a ; Norma Angé ; lica Montes-Colima^a ; Marí ; a Asunci&oacute ; n Moreno-Pé ; rez^a ; Adriana Guadalupe Galicia-Nicolá ; s^a ; Irma L&oacute ; pez-Martí ; nez^a ; Cuitlá ; huac Ruiz-Matus^c ; Pablo Kuri-Morales^d ; Joanna Marí ; a Ortí ; z-Alcá ; ntara^a ; Fabiola Garcé ; s-Ayala^a ; José ; Ernesto Ramí ; rez-Gonzá ; lez^a
• 关键词：Cholera ; Vibrio cholerae ; Phylogenomics ; Disease control ; PFGE
• 刊名：Microbes and Infection
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：18
• 期：5
• 页码：322-328
• 全文大小：1111 K

文摘

The first week of September 2013, the National Epidemiological Surveillance System identified two cases of cholera in Mexico City. The cultures of both samples were confirmed as Vibrio cholerae serogroup O1, serotype Ogawa, biotype El Tor. Initial analyses by PFGE and by PCR-amplification of the virulence genes, suggested that both strains were similar, but different from those previously reported in Mexico. The following week, four more cases were identified in a community in the state of Hidalgo, located 121 km northeast of Mexico City. Thereafter a cholera outbreak started in the region of La Huasteca. Genomic analyses of the four strains obtained in this study confirmed the presence of Pathogenicity Islands VPI-1 and -2, VSP-1 and -2, and of the integrative element SXT. The genomic structure of the 4 isolates was similar to that of V. cholerae strain 2010 EL-1786, identified during the epidemic in Haiti in 2010.