Vaccination of cattle with a recombinant bivalent toxoid against botulism serotypes C and D
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- 作者：Carlos E.P. Cunha ; Gustavo M.S.G. Moreira ; Felipe M. Salvarani ; Monique S. Neves ; Francisco C.F. Lobato ; Odir A. Dellagostin ; Fabricio R. Concei莽茫o
- 关键词：Recombinant vaccine ; Fused antigens ; Clostridium botulinum ; Botulinum neurotoxins ; LTB
- 刊名：Vaccine
- 出版年：3 January, 2014
- 年：2014
- 卷：32
- 期：2
- 页码：214-216
- 全文大小：315 K
文摘
Cattle botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum serotypes C and D resulting in economic losses. Vaccination is the most effective way to control botulism. However, the commercially available vaccines are difficult and hazardous to produce. Neutralizing antibodies against the C-terminal fragment of the BoNT heavy chain (HC) are known to protect against lethal doses of BoNTs. We report the vaccination of cattle with a previously tested recombinant chimera consisting of Escherichia coli heat-labile enterotoxin B subunit and the HC of BoNTs C and D. Vaccinated animals produced neutralizing antibodies against serotypes C and D averaging 5 卤 0 and 6.14 卤 1.06 IU/mL, respectively. For BoNT D, the titers were greater than those measured for the commercial vaccine, which induced titers of 5 卤 0 and 2.85 卤 1.35 against the respective serotypes, suggesting that this chimera is effective against cattle botulism.