Colocalization of neutrophils, extracellular DNA and coagulation factors during NETosis: Development and utility of an immunofluorescence-based microscopy platform
详细信息 查看全文
- 作者：Laura D. Healya ; healyl@ohsu.edu" class="auth_mail" title="E-mail the corresponding author ; Cristina Puyb ; Asako Itakuraa ; Tiffany Chub ; David K. Robinsonb ; Alan Bylundb ; Kevin G. Phillipsb ; Elizabeth E. Gardinerc ; Owen J.T. McCartya ; b
- 关键词：NETs ; neutrophil extracellular traps ; FXII ; factor XII ; FXI ; factor XI ; FX ; factor X ; FX-GD ; Gla-less FX ; FVIIa ; factor VIIa ; APC ; Activated protein C ; APC-GD ; Gla-less APC ; PMA ; phorbol 12-myristate 13-acetate ; PKC ; protein kinase C ; Gla-domain ; γ-carboxyglutamic acid domain ; HK ; high molecular weight kininogen ; AnxV ; Annexin V
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：435
- 期：Complete
- 页码：77-84
- 全文大小：1146 K
文摘
- •
The coagulation factor fibrinogen binds DNA-rich NETs.
- •
Coagulation factors prothrombin, FX and FVIIa bind activated neutrophils during NETosis.
- •
The anticoagulant activated protein C (APC) binds activated neutrophils and DNA-rich NETs.
- •
Binding of FX and APC to activated neutrophils is dependent on the Gla-domain and exposure of phosphatidylserine.