Identification of a Δ12 fatty acid desaturase from oil palm (Elaeis guineensis Jacq.) involved in the biosynthesis of linoleic acid by heterologous expression in Saccharomyces cerevisiae
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- 作者：Ruhao Sun1 ; Lingchao Gao1 ; Xiaoping Yu ; Yusheng Zheng ; Dongdong Li ; liddfym@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Xinguang Wang ; xg-wang@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：ALA ; α-linolenic acid ; CTAB ; cetyltrimethylammonium bromide ; DAP ; days after pollination ; EFAs ; essential fatty acid ; ER ; endoplasmatic reticulum ; FAD2 ; Δ12 fatty acid desaturase ; FAMEs ; fatty acid methyl esters ; GC&ndash ; MS ; gas chromatograph-mass spectrometer-computer ; GLA ; γ-linolenic acid ; LA ; linoleic acid ; LC-PUFA ; long-chain polyunsaturated fatty acid ; RACE ; rapid amplification of cDNA ends ; RT&ndash ; PCR ; real-time fluorescent quantitative polymerase chain reaction
- 刊名：Gene
- 出版年：2016
- 出版时间：10 October 2016
- 年：2016
- 卷：591
- 期：1
- 页码：21-26
- 全文大小：1490 K
文摘
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A Δ12-desaturases associated with LC-PUFA biosynthesis were cloned from oil palm (Elaeis guineensis Jacq).
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The yield of egFAD2 was the highest in the mesocarp of oil palm fruits at 120-140 DAP.
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Heterologous expression of egFAD2 in Saccharomyces cerevisiae produced an appreciable amount of linoleic acids.
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These results indicated that the egFAD2 was able to converted oleic acid to linoleic acid but did not accept palmitoleic acid as a substrate.