Molecular basis of thermostability enhancement of Renilla luciferase at higher temperatures by insertion of a disulfide bridge into the structure

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• 作者：Zahra Fanaei Kahrani^a ; Rahman Emamzadeh^a ; ^{Sci_rahman@yahoo.com} ; ^{r.emamzadeh@sci.ui.ac.ir} ; Mahboobeh Nazari^b ; Seyed Mohammad Mahdi Rasa^a
• 关键词：Super Renilla luciferase 8 (SRLuc 8) ; Kinetic stability ; Disulfide bridge ; Molecular dynamic simulation
• 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1865
• 期：2
• 页码：252-259
• 全文大小：1179 K
• 卷排序：1865

文摘

An engineered Renilla luciferase, C-SRLuc 8 namely, was obtained by insertion of a disulfide bridge into protein structure. C-SRLuc 8 showed a significant increase in thermostability at 65°C. The relationship between the protein structure, activity and thermostability was studied in C-SRLuc 8 using MD simulations.