An eco-friendly, simple, and sensitive fluorescence biosensor for the detection of choline and acetylcholine based on C-dots and the Fenton reaction

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• 作者：Jianfei Wei ; Jun Ren ; Jing Liu ; Xianwei Meng ; Xiangling Ren ; Zhenzhen Chen ; Fangqiong Tang
• 关键词：Carbon dots ; Fluorescence ; Choline ; Acetylcholine ; Fenton reaction
• 刊名：Biosensors and Bioelectronics
• 出版年：15 February, 2014
• 年：2014
• 卷：52
• 期：Complete
• 页码：304-309
• 全文大小：3232 K

文摘

A simple and novel method is proposed for the preparation of Carbon dots (C-dots) with excellent properties. We firstly demonstrated that the fluorescence of C-dots decreased apparently in the presence of H₂O₂ and Fe²⁺. Based on the this finding, C-dots are successfully adopted as probes for the detection of H₂O₂. After the experimental conditions are optimized, the limit of detection (LOD) for H₂O₂ is found to be 0.1 渭M. Furthermore, we established an eco-friendly, simple and sensitive biosensor for the detection of choline and acetylcholine (ACh) based on the detection of H₂O₂ using C-dots as probes. The detection limit for choline is 0.1 渭M and the linear range is 0.1-40 渭M. The detection limit for ACh is found to be 0.5 渭M and the linear range is 0.5-60 渭M. The excellent performance of the proposed biosensor shows that this method possesses the potential for practical application.