miR-137 suppresses cell growth in ovarian cancer by targeting AEG-1

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• 作者：Jinling Guo ; Bairong Xia ; Fanling Meng ; Ge Lou
• 关键词：AEG-1 ; miR-137 ; Ovarian cancer ; Cell growth
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：15 November, 2013
• 年：2013
• 卷：441
• 期：2
• 页码：357-363
• 全文大小：1612 K

文摘

Astrocyte elevated gene-1 (AEG-1) is an oncogene overexpressed in multiple types of human cancers including ovarian cancer (OC). However, the underlying mechanism of AEG-1 up-regulation in OC is not well understood. In this study, we showed that miR-137 downregulated AEG-1 expression through interaction with its 3鈥?untranslated region (3鈥睻TR) and that miR-137 expression was inversely correlated with AEG-1 levels in OC specimens. Similar to the downregulation of AEG-1, overexpression of miR-137 in OC cell lines decreased in vitro cell growth, clonogenicity, and also induced G1 arrest. Importantly, miR-137 overexpression suppressed in vivo tumor growth in nude mice models. Furthermore, we found that restoring the AEG-1 (without the 3鈥睻TR) significantly rescued miR-137-induced cell growth inhibition and cell-cycle arrest. Taken together, these findings indicate that miR-137 functions as a tumor suppressor by inhibition of AEG-1. These molecules might be targets for prevention or treatment of OC.