- 作者：Juliane Maria Guerreiro-Tanomaru ; DDS ; PhD&lowast ; ; Norberto Batista de Faria-Jú ; nior ; DDS ; PhD&lowast ; ; Marco Antô ; nio Hú ; ngaro Duarte ; DDS ; PhD&dagger ; ; Ronald Ordinola-Zapata ; DDS ; MSc&dagger ; ; Má ; rcia Sirlene Zardin Graeff ; MSc&Dagger ; ; Má ; rio Tanomaru-Filho ; DDS ; PhD&lowast ; ; tanomaru@foar.unesp.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Biofilm ; confocal microscopy ; Enterococcus faecalis
- 刊名：Journal of Endodontics
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：39
- 期：3
- 页码：346-350
- 全文大小：749 K
Methods
Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm3), live bacteria biovolume (μm3), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05).
Results
Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05).
Conclusions
E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated.