Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin

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• 作者：Vladimir Ló ; pez^a ; David Gonzá ; lez-Barrio^a ; José ; Francisco Lima-Barbero^b ; José ; Antonio Ortiz^c ; Lucas Domí ; nguez^d ; Ramó ; n Juste^e ; Joseba M. Garrido^e ; Iker A. Sevilla^e ; Pilar Alberdi^a ; José ; de la Fuente^a ; ^f ; Christian Gortá ; zar^a ; ^{christian.gortazar@uclm.es" class="auth_mail" title="E-mail the corresponding author} ; ^{christian.gortazar@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Animal tuberculosis ; Complement component 3 ; Intradermal tuberculin test ; Ruminants ; Serum antibodies
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：172
• 期：Complete
• 页码：21-25
• 全文大小：413 K

文摘

Orally delivered mycobacterial antigens may not sensitize the immunized animals causing a positive tuberculin skin test response. As the first step to address this critical issue, we characterized the response of farmed red deer (Cervus elaphus) to orally delivered heat-inactivated Mycobacterium bovis. Thirty-two adult red deer hinds from a farm known to be free of tuberculosis (TB) were randomly assigned to two different treatment groups, immunized (n = 24) and control (n = 8). Immunized hinds were dosed orally with 2 ml of PBS containing 6 × 10⁶ heat-inactivated M. bovis. The mean skin test response of immunized deer to both avian purified protein derivative (aPPD) and bovine PPD (bPPD) was consistently lower in immunized than in control hinds. One year after immunization, immunized hinds had a significant reduction in the skin test response to aPPD and in the ELISA antibody levels against both aPPD and bPPD (24–36% reduction; P < 0.05). By contrast, no significant change was observed in the skin test response to phytohaemagglutinin, or in the ELISA antibody levels against the M. bovis specific antigen MPB70. The mRNA levels for C3, IFN-γ and IL-1β and serum protein levels for IFN-γ and IL-1β did not vary between immunized and control deer. However, serum C3 protein levels were significantly higher (P = 0.001) in immunized than in control deer six months after immunization. These results confirm that oral heat-inactivated M. bovis does not sensitize farmed red deer and therefore does not cause false-positive responses in the tuberculin skin test. The absence of sensitization in orally immunized deer opens the possibility of testing the vaccine in deer and possibly other ruminants without the risk of causing false-positive reactions in TB-tests. This study also provided the first evidence that orally-delivered inactivated mycobacterial antigens elicit some kind of immune response in a ruminant.