Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus

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• 作者：Abolfazl Fateh^a ; ^b ; Mohammadreza Aghasadeghi^c ; Seyed D. Siadat^a ; Farzam Vaziri^a ; Farzin Sadeghi^d ; Roohollah Fateh^e ; Hossein Keyvani^f ; Alireza H. Tasbiti^a ; Shamsi Yari^a ; Angila Ataei-Pirkooh^f ; Seyed H. Monavari^f ; ^{afateh2@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：amplification refractory mutation system (ARMS)-PCR ; direct DNA sequencing ; Hepatitis C ; real-time polymerase chain reaction (PCR)
• 刊名：Osong Public Health and Research Perspectives
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：7
• 期：2
• 页码：83-89
• 全文大小：442 K

文摘

This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.

Methods

A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.

Results

The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).

Conclusion

Screening by ARMS – PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.