Proteomics as a tool to investigate cell models for dopamine toxicity

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• 作者：Mauro Fasano ; Tiziana Alberio ; Monica Colapinto ; Silvia Mila ; Leonardo Lopiano
• 关键词：Dopamine ; Human neuroblastoma ; Human T-cell leukaemia ; Parkinson's disease ; Proteomics ; α ; -Synuclein
• 刊名：Parkinsonism and Related Disorders
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：14
• 期：Supplement 2
• 页码：S135-S138
• 全文大小：204 K

文摘

Dopaminergic neurons are particularly exposed to oxidative stress because dopamine metabolism gives rise to endogenous toxins. Here, two different models for dopamine toxicity have been investigated using proteomics: stable α-synuclein-expressing neuroblastoma (SH-SY5Y) cells to understand how α-synuclein modulates dopamine toxicity at the central level, and cultured T-cell leukaemia (Jurkat) cells challenged with dopamine or l-dopa to evaluate protein changes on the surface of peripheral cells. α-synuclein-expressing cells were more resistant compared with wild-type cells, showing upregulation of antioxidant proteins including the Parkinson's disease-related DJ-1 protein. In addition, treatment of prototypical T-cells with l-dopa induced significant changes in proteins on the cell surface, indicating that peripheral blood lymphocytes may be good indicators of dopamine toxicity.