Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes
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- 作者：Takuya Shorikia ; b ; Madoka Ichikawa-Sekia ; b ; madoka@iwate-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Keisuke Suganumac ; Ikunori Naitod ; Kei Hayashia ; b ; Minoru Nakaoe ; Junya Aitaa ; b ; Uday Kumar Mohantaa ; b ; Noboru Inouec ; Kenji Murakamid ; Tadashi Itagakia ; b
- 关键词：Fasciola spp. ; pepck ; pold ; PCR&ndash ; RFLP ; Multiplex PCR
- 刊名：Parasitology International
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：65
- 期：3
- 页码：180-183
- 全文大小：534 K
文摘
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Precise and robust methods to discriminate Fasciola spp. have been required.
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PCR–RFLP and multiplex PCR were successfully developed by using single copy genes.
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Fasciola hepatica, F. gigantica, and aspermic Fasciola flukes were distinguished.
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Aspermic flukes had a mixed fragment pattern of F. hepatica and F. gigantica.
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Aspermic flukes were revealed as descendants of interspecific hybridization.