Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

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• 作者：Mohammad Saraji ; Fatemeh Mousavi
• 关键词：Liquid– ; liquid– ; liquid microextraction ; Liquid chromatography ; Food analysis ; Phenolic acids
• 刊名：Food Chemistry
• 出版年：2010
• 出版时间：15 December 2010
• 年：2010
• 卷：123
• 期：4
• 页码：1310-1317
• 全文大小：457 K

文摘

This paper describes a simple method based on three-phase hollow-fibre liquid-phase microextraction (HF-LPME) for the determination of phenolic acids in fruit juices. Analytes including gallic acid, p-hydroxy benzoic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, and cinnamic acid were separated and determined using high-performance liquid chromatography–photodiode array detection (HPLC–DAD). Parameters affecting the enrichment factors (EFs) were investigated. These compounds were extracted from 5 mL aqueous samples (pH 2) to a thin layer of organic solvent (hexyl acetate) phase impregnated into the pores of the polypropylene hollow fibre wall, and then back extracted to a basic acceptor solution (0.02 M NaOH). EFs ranged from 15 (gallic acid) to 408 (cinnamic acid). The RSD of the method for the analysis of spiked water and fruit juice samples varied from 3.1 % to 11.3 % . The LODs ranged from 0.01 (cinnamic acid) to 2.0 (caffeic acid) μg/L.