TR4 activates FATP1 gene expression to promote lipid accumulation in 3T3-L1 adipocytes

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• 作者：Hojung Choi^a ; Seung-Jin Kim^a ; Sung-Soo Park^a ; Chawnshang Chang^b ; Eungseok Kim^a ; ^{ekim@chonnam.ac.kr}
• 关键词：TR4 ; FATP1 ; Adipocytes ; Fatty acid uptake ; Lipid accumulation
• 刊名：FEBS Letters
• 出版年：2011
• 出版时间：2 September, 2011
• 年：2011
• 卷：585
• 期：17
• 页码：2763-2767
• 全文大小：1K

文摘

We show that TR4 facilitates lipid accumulation in 3T3-L1 adipocytes via induction of the FATP1 gene. Further study showed that TR4 transactivated FATP1 5?promoter activity via direct binding to the TR4 responsive element located at the FATP1 5?promoter region. Constitutive overexpression of TR4 in 3T3-L1 adipocytes resulted in increased lipid accumulation, accompanied by an increase in fatty acid uptake. However, small interfering RNA knockdown of FATP1 abolished TR4-enhanced fatty acid uptake. Moreover, microRNA-mediated silencing of TR4 in 3T3-L1 adipocytes drastically reduced basal FATP1 5?promoter activity and FATP1 expression with reduced lipid accumulation.