The binding modes of TNP-AMP into the human liver FBPase has been re-examined.
Not only AMP but also FBP can competitively inhibit the binding of TNP-AMP to FBPase.
TNP-AMP can bind to both the active site and the allosteric site.
K274 is important for TNP-AMP binding to the active site of FBPase.
K274L is good candidate for exploring the allosteric site with TNP-AMP as fluorescent probe.