Inversed relationship between CD44 variant and c-Myc due to oxidative stress-induced canonical Wnt activation

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• 作者：Go J. Yoshida ; Hideyuki Saya
• 关键词：Cancer stem-like cell ; CD44 variant ; Redox stress-induced Wnt signal ; c-Myc ; Fbw7 ; Gastric cancer
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：10 January, 2014
• 年：2014
• 卷：443
• 期：2
• 页码：622-627
• 全文大小：1927 K

文摘

Cancer stem-like cells express high amount of CD44 variant8-10 which protects cancer cells from redox stress. We have demonstrated by immunohistochemical analysis and Western blotting, and reverse-transcription polymerase chain reaction, that CD44 variant8-10 and c-Myc tend to show the inversed expression manner in gastric cancer cells. That is attributable to the oxidative stress-induced canonical Wnt activation, and furthermore, the up-regulation of the downstream molecules, one of which is oncogenic c-Myc, is not easily to occur in CD44 variant-positive cancer cells. We have also found out that CD44v8-10 expression is associated with the turn-over of the c-Myc with the experiments using gastric cancer cell lines. This cannot be simply explained by the model of oxidative stress-induced Wnt activation. CD44v8-10-positive cancer cells are enriched at the invasive front. Tumor tissue at the invasive area is considered to be composed of heterogeneous cellular population; dormant cancer stem-like cells with CD44v8-10 ^high/ Fbw7 ^high/ c-Myc ^low and proliferative cancer stem-like cells with CD44v8-10 ^high/ Fbw7 ^low/ c-Myc ^high.